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CCNG2 in vivo tumor formation from U251 cells in nude mice. Stable CCNG2 transfected and empty vector U251 cells were implanted subcutaneously in the left flank of nude mice. (A) After 42 days, mice were sacrificed and tumors were excised. (B) Effect of CCNG2 on tumor volume in a nude mice model. Apoptosis and Ki67 expression were detected by (C) TUNEL and (D) immunohistochemistry assays. Scale bars: 100 µm. ***P < 0.001 by the unpaired two-tailed Student’s t-test.
