Figure 5. AXL blocks binding of c-ABL to p73β protein and attenuates CDDP-induced phosphorylation of c-ABL.
A) OE33 cells stably expressing AXL or pcDNA4 were treated with vehicle or CDDP (10 μmol/L) for 48h. The Western blot data indicate that CDDP treatment significantly increased p-c-ABL(Y412), p-p73β(Y99), and p73β protein levels in control cells. In contrast, AXL expression abrogated these effects in response to CDDP. B) Western blot analysis of immunoprecipitated exogenous p73β protein with M2-flag antibody in OE33 cells transfected with p73β-Flag alone or in combination with AXL. Exogenous p73β interacted with endogenous c-ABL protein, and the p73β/c-ABL protein complex was disrupted by AXL. C) Cell viability of OE33 cells transfected with pcDNA4 alone, AXL alone or in combination with GFP-c-ABL was assessed 48h post-transfection. AXL expression blocked c-ABL-induced cell death. D) Western blot analysis of c-ABL and AXL proteins in OE33 cells transiently transfected as in panel C.