Fig. 6.
The surface composition and size of LNPs regulates cellular production. (A) Area per DSPC molecule and (D) number of mRNA per LNP as a function of the LNP size for LNPs with variable (blue) and constant (purple) surface composition. The horizontal line corresponds to the reported value of the area per DSPC in the gel phase (26). (B and E) Number of hEPO produced per mRNA dosed as a function of time in (B) adipocytes and (E) hepatocytes for LNPs with constant surface composition and different size: <d>N = 47 nm (red circles), <d>N = 64 nm (green squares), <d>N = 99 nm (yellow triangles), and <d>N = 133 nm (blue diamonds). (C and F) Number of hEPO expressed per mRNA dosed after 48 h of dosing (C) adipocytes and (F) hepatocytes for LNPs with variable (blue) and constant (purple) surface composition. Lines are to guide the eye. The experiments were done in the presence of 1% human serum. Values are means ± SEM (n = 3).