. 2018 Mar 9;7:e35074. doi: 10.7554/eLife.35074

Table 1. Nur77-eGFP is a specific reporter of antigen-dependent signaling in vivo.

Conclusion	Stimulus/perturbation	Pathway	Readout	Cell type	References
Does not modulate Nur77 at steady state in vivo	CD40L^-/-	CD40	Nur77-eGFP	B cells	Manuscript Figure 1—figure supplement 1A
	TLR7^-/-	TLR7	Nur77-eGFP	B cells	Manuscript Figure 1—figure supplement 1A
	Un93b1^3d/3d	TLR3/7/9	Nur77-eGFP	B cells	Manuscript Figure 1—figure supplement 1A
	MyD88^fl/fl MB1-Cre	MyD88	Endog. Nur77 protein/transcript	PerC B1a cells/spleen	Manuscript Figure 1—figure supplement 1F,G
	Germ-free mice	MyD88/TRIF	Endog. Nur77 protein/transcript	Splenic B cells/spleen	Manuscript Figure 1—figure supplement 1D,E
	Const. act. STAT5	Jak/Stat	Nur77-eGFP	Thymocytes	Moran et al. JEM 2011, Figure 8.
Does not induce Nur77 in vitro	BAFF	BAFFR	Nur77-eGFP	B cells	Zikherman et al. (2012): Figure S1G
	IL-4	Jak/Stat	Nur77-eGFP	B cells	Manuscript Figure 1—figure supplement 1B
	IL-2, IL-15	Jak/Stat	Nur77-eGFP	CD8 (IL-2, 15), CD4 (IL-2)	Au-Yeung et al. JI 2017, Figures S1A, 3C, 4A
	CXCL12/ SDF-1	CXCR4	Nur77-eGFP	B cells	Manuscript Figure 1—figure supplement 1C
Induces Nur77 in vitro but does not require IgM or IgD specifically	LPS	TLR4, Rp150	Nur77-eGFP	B cells	Zikherman et al. (2012): Figure S1G; Manuscript Figure 1—figure supplement 3A
	CpG	TLR9	Nur77-eGFP	B cells	Zikherman et al. (2012), Figure S1G; Manuscript Figure 1—figure supplement 3A
	Pam3CSK4	TLR1/2	Nur77-eGFP	B cells	Manuscript Figure 1—figure supplement 3A
	Anti-Igκ	BCR	Nur77-eGFP	B cells	Manuscript Figure 2F
Modulates pathway at steady state in vivo	IgHEL Tg	Antigen/BCR	Nur77-eGFP	B cells	Zikherman et al. (2012), Figure 3B,C; Manuscript Figure 1A
	IgHEL BCR Tg/sHEL Ag	Antigen/BCR	Nur77-eGFP	B cells	Zikherman et al. (2012), Figure 3B,C; Manuscript Figure 1A
	Lyn^-/-	BCR via ITIMs	Nur77-eGFP	B cells	Manuscript Figure 5—figure supplement 3A
	CD45 allelic series	BCR via SFKs	Nur77-eGFP	B cells	Zikherman et al. (2012), Figure 3A,B