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. 2018 Mar 9;7:e35074. doi: 10.7554/eLife.35074

Author response image 3. Self-reactive B cells upregulate Nur77-eGFP at early stages of B cell development.

Author response image 3.

Nur77-eGFP BAC Tg was crossed to the Vh3H9 HC site directed Tg. BM and splenic cells from these mice (red) and unrestricted repertoire reporter mice (gray) were harvested and stained ex vivo to identify B cell subsets as well as surface light chain expression. Histograms depicting GFP expression in K+ and L+ B cells subsets are representative of > 9 biological replicates.