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. 2018 Mar 9;7:e35074. doi: 10.7554/eLife.35074

Figure 4. Cell-intrinsic skewing of B cell development by IgM and IgD BCRs.

(A) Allelic exclusion leads to a 1:1 mixture of IgM-only and IgD-only B cells in IgM+/− IgD−/+ mice. (B) Proportion of peritoneal B1a (CD19+CD5+CD23-) and splenic MZ (B220+CD21hiCD23lo) B cells originating from each Ig locus in IgM+/− IgD−/+ mice. (C) Relative competition between IgM+ and IgD+ B cells in IgM+/− IgD−/+ mice was calculated for bone marrow, splenic, and peritoneal B cell compartments. Results include data from (B) for reference. Immature (CD23-CD93+); T2-like (CD23+CD93+); mature recirculating (CD23+CD93-); T1 (CD93+CD23-); T2/3 (CD93+CD23+); Fo (CD93-CD23+); MZ (CD21hiCD23lo); B1a (CD5+CD23-); B1b (CD5-CD23-); B2 (CD5-CD23+). (D) Relative competition between WT (IgMb+) and IgM-null (IgDa+) B cells in IgM+/− mice was determined as in (C). (E) Relative competition between WT (IgMb+) and IgD-null (IgMa+) B cells in IgD+/− mice was determined for splenic and peritoneal compartments as described in (C). (F) Competition in peritoneal B1a and splenic MZ compartments in IgM+/− mice with or without a BAFF overexpression transgene. Results include data from (D) for reference. For (B) and (C), n = 3–5 mice were analyzed. For (D), n = 3–8 mice were analyzed. For (E), n = 5 mice were analyzed. For (F), n = 4–5 mice of each genotype were analyzed. Welch’s t test was used to calculate p values, and mean ±SEM is displayed. **p<0.01, ***p<0.001.

Figure 4—source data 1. Numerical data corresponding to competition between IgM-only, IgD-only and WT B cells in Figure 4B–F, and in Figure 4—figure supplement 1B-D.
Numerical data corresponding to competition between IgM-only, IgD-only and WT B cells inFigures 4B-F.Numerical data corresponding to splenic and peritoneal B cell compartment sizes in Figure 4—figure supplement 1B–C. Numerical data corresponding to competition between IgHa and IgHb B cells in Figure 4—figure supplement 1D.
elife-35074-fig4-data1.zip (127.7KB, zip)
DOI: 10.7554/eLife.35074.021
Figure 4—source data 2. Numerical data corresponding to competition between IgM-only, IgD-only and WT B cells in Figure 4B–E.
elife-35074-fig4-data2.xlsx (49.2KB, xlsx)
DOI: 10.7554/eLife.35074.022
Figure 4—source data 3. Numerical data corresponding to competition between IgD-only and WT B cells in Figure 4F.
elife-35074-fig4-data3.xlsx (41.5KB, xlsx)
DOI: 10.7554/eLife.35074.023

Figure 4.

Figure 4—figure supplement 1. B cell subset development in IgM−/−, IgD−/−, WT, and IgHa/b mice.

Figure 4—figure supplement 1.

(A) Signal strength model of B1a and MZ B cell development. While B1a and MZ cells originate from different precursor populations, their development is thought to be BCR signal strength-dependent. (B) B cell compartments as a percentage of total B220+ splenic B cells. T1 (CD93+CD23-); T2/3 (CD93+CD23+); Fo (CD93-CD23+); MZ (CD21hiCD23lo). (C) B cell compartments as a percentage of total CD19+ peritoneal B cells. B1a (CD5+CD23-); B1b (CD5-CD23-); B2 (CD5-CD23+). (D) Relative competition between IgMa+ and IgMb+ B cells in Balb/c-B6 F1 mice was calculated for splenic and peritoneal compartments described in (B–C). For (B), n = 10–12 mice of each genotype were analyzed. For (C), n = 6 mice of each genotype were analyzed. For (D), n = 3 mice were analyzed. Mice in (D) were 6–14 weeks old. One-way ANOVA with Tukey’s multiple comparisons test (B-C) was used to calculate p values, and mean ±SEM is displayed. *p<0.05, **p<0.01, ***p<0.001.