Extended Data Figure 1. Conditioned medium generated from UV or ionizing irradiation (IR) and purified tCPR-4 proteins cause embryonic lethality.
a, The embryonic lethality rate of wild type (N2) or cep-1(gk138) animals after 100 J/m2 UV irradiation or 500 Gy IR compared with sham-irradiation controls. b, N2 animals were used to generate UV-CM, UV-control, IR-CM and IR-control, which were used to treat unexposed N2 animals in the embryonic lethality assays (Methods). c, 2.8 μM of recombinant tCPR-4 proteins (wild-type or mutant), 0.27 μM recombinant human Cathepsin B (rhCTSB), or the buffer control were used to treat N2 animals in the embryonic lethality assays. Total numbers of embryos scored: 1781, 805, 1249, 2645, 596, and 1862 embryos, from the left bar to the right bar in a; 2721, 2484, 880, and 743, from left to right in b; and 979, 875, 929, 939, 907, and 777, from left to right in c. Six independent assays (a, UV-Ctrl and UV-CM in b) and three independent assays (IR-Ctrl and IR-CM in b, c) were performed for each condition. Data are mean ± s.e.m. **P <0.01, ***P <0.001, “ns” is non-significant, two-sided, unpaired t test.