Extended Data Figure 6. Analysis of the roles of additional genes in mediating RIBE.
a, Localized UV irradiation assays. Animals of the indicated genotype were analyzed for the bystander Phsp-4::gfp response 24 hours post localized irradiation at the head region as described in Fig. 3. Data are mean ± s.e.m. The numbers of animals scored are indicated inside the bars. b, Germ cell corpse assays after tCPR-4 treatment. 2.8 μM recombinant tCPR-4 protein or buffer control was used to treat L4 larvae of the indicated genotype as described in Fig. 4a. Data are mean ± s.e.m. The numbers of gonad arms scored are indicated inside the bars. c, Immunoblotting analysis of secreted CPR-4::Flag in UV-CM and UV-Ctrl from Pcpr-4::cpr-4::flag; daf-2(e1730); cpr-4(tm3718) animals was done as in Fig. 1f. d, Germ cell corpse assays. ced-1(e1735) L4 larvae were treated with UV-CM and UV-control (0.1 μg/μL) prepared from c. Data are mean ± s.e.m. The numbers of gonad arms scored are indicated inside the bars. e, Germ cell proliferation assays. N2 and cep-1(gk138) L4 larvae were treated in S-Medium containing 2.8 μM of recombinant tCPR-4 or buffer control for 48 hours. The numbers of nuclei and metaphase nuclei in the mitotic zone of the germline were scored (Methods). Data are mean ± s.e.m. In a, b, d, e, * P <0.05, ** P < 0.01, *** P < 0.001, “ns”, non-significant, two-sided, unpaired t test. For gel source data, see Supplementary Fig. 1.