Figure 6. HuR is a target of EV-derived miR-146a.
(A-B) HuR mRNA (A) and protein (B) expression in BMDMs transfected with control anti-miR/anti-miR146a and control mimic/miR-146a mimic. (C) Co-culture of miR-146a transfected macrophages in the absence/presence of oxLDL for 72hs with naïve macrophages resulted in an increased expression of miR-146a and a decreased expression of HuR in naïve cells. (D-E) Knockdown of HuR using siRNA reduces macrophage migration. BMDMs were transfected with control or HuR siRNA for 72h. The migration of transfected cells towards CCL2 (150 ng/ml) was measured using the xCELLigence System. All graphs in this figure represent the means ± SD from n=3 independent experiments, *p<0.05, **p <0.01. Western blot is representative of a single experiment done in triplicate (n=3).