Figure 2.
All NLRP3 inflammasome components were constitutively expressed, and upregulated and activated in IL-10-/- mice. [A] Western blot analyses of inflammasome proteins in colonic mucosa from wild type [WT] or IL-10-/- mice with or without macroscopic colitis [MD] at different ages [1, 2, and 3 months old]. Total lysate [60 μg] was analysed by sodium dodecyl sulphate polyacrylamide gel electrophoresis [SDS-PAGE] and western blot with antibodies indicated. The two lanes in pairs in each column [labelled as 1 and 2] are samples from two individual mice. Representatives of at least four mice in each group are shown. [B] Transcriptional regulation of NLRP3 inflammasome. The mRNA of each component was analysed by real-time polymerase chain reaction [RT-PCR] from colonic mucosa of 3-month-old mice. Note: The 1- and 2-month-old no-macroscopic-colitis IL-10-/- mice in this study exhibited neither macroscopic nor microscopic colitis. Casp-1/p20 and IL-1β/p17 are the active forms of caspase-1 and IL-1β. Representatives of data obtained from experiments with 3–5 mice are shown; *p < 0.05.