Figure 2.
Phylogeny of enolase. Numbers at nodes correspond to bootstrap support over 50% for major nodes from neighbor-joining (upper number) and Fitch–Margoliash (lower number) analyses. Groups of related organisms are named on the right, and the names of those with the two insertions highlighted in Fig. 1 are circled. Nodes where alveolates were positioned in partially unresolved trees and were tested by Kishino–Hasegawa tests are indicated as follows: ●, positions rejected at the 5% level (many were rejected at the 1% level—see text); ○, positions not rejected. The position of alveolates is not well supported by bootstrap analysis (and within alveolates, ciliates are not monophyletic), but their separation from land plants and charophytes is supported strongly by Kishino–Hasegawa tests (see text).