Fig. 7. Molecular interference of miR-125a-5p and miR-16-5p prevents ADEV-IL-1β from reducing dendritic arborization.
a Representative fluorescent images of MAP2 immunopositive hippocampal neurons (top panels) and Neurolucida dendrite tracings (bottom panels) following a 48 h exposure to the indicated treatment conditions. Quantitative data show (b) dendritic complexity, c surface area, d dendritic length, e number of ends, f nodes, and g total dendrite number for the indicated treatment conditions. ADEV-IL-1β particle dose was 50 ADEVs/cell, and antisense oligonucleotide inhibitors for miR-125 and miR-16 were used at 20 pmole. Data are mean ± SEM for the indicated treatment conditions. One-way ANOVA followed by Tukey’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001 increased compared to NT. #p < 0.05, ##p < 0.01, ###p < 0.001 decreased compared to NT