Figure 5.
Acinetobacter baumannii inhibiting NET formation by suppressing neutrophil adhesion. (A) Neutrophils were stimulated with 200-nM phorbol 12-myristate 13-acetate (PMA) and cocultured with A. baumannii (MOI 50) for 30 min. The non-adhering neutrophils were removed, the adhering cells were lysed, and LDH levels determined. The adhesion rate was calculated as a percentage compared with lysed neutrophils. (B) Neutrophils were cultured for 3 h in poly-HEMA-coated plates (poly-HEMA inhibits cell adhesion), in the absence or presence of 200-nM PMA, and A. baumannii (MOI 50). Extracellular DNA was stained with SYTOX green and the signal quantified. The data are shown as the mean ± SD; n ≥ 3 per group. ***p < 0.001. The results are representative of at least three experiments.