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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: Cell Stem Cell. 2018 Feb 1;22(2):206–220.e4. doi: 10.1016/j.stem.2017.12.017
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
Rabbit monoclonal anti-Olfm4 Cell Signaling Cat# 39141S; RRID: AB_2650511
Rabbit polyclonal anti-Lysozyme Dako Cat# A0099; RRID: AB_2341231
Rabbit polyclonal anti-Chromogranin A ImmunoStar Cat# 20086, RRID: AB_572226
Rabbit polyclonal anti-cleaved caspase 3 Cell Signaling Cat# 9661, RRID: AB_2341188
Rabbit monoclonal anti-Id1 BioCheck Cat# BCH-1/37-2, RRID:AB_2713996
Rat monoclonal anti-Ly-6G BioLegend Cat# 127601, RRID:AB_1089179
Rat monoclonal anti-F4/80 BioLegend Cat# 123101, RRID:AB_893504
Rabbit monoclonal anti-SREBP2 EMD Millipore Cat#: MABS1988
F(ab)2-Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 555 Thermo Fisher Scientific Cat# A-21430 RRID:AB_2535851
R.T.U. Biotinylated Goat Anti-Rat IgG Antibody Vector laboratories BP-9400
DAKO EnVision+ Single Reagents (HRP. Rabbit) Agilent K400311-2
Chemicals, Peptides, and Recombinant Proteins
Tamoxifen Sigma-Aldrich T5648
Matrigel Fisher CB-40230C
JAG-1 Anaspec. AS-61298
EGF Recombinant Mouse Protein Thermofisher PMG8041
GlutaMAX™ Supplement Thermofisher 35050-061
Noggin Peprotech 250-38
R-spondin Thermo Fisher Scientific 4645-RS-025
Y-27632 Sigma-Aldrich Y0503-1MG
N-Acetyl-L cysteine Sigma-Aldrich A7250-5g
B27 Thermo Fisher Scientific 12587-010
N2 Thermo Fisher Scientific 17502-048
4-hydroxytamoxifen Sigma H7904
Simvastatin EMD Biosciences 567021-5MG
Ro 48-8071 Cayman Chemical 10006415
Methyl-β-cyclodextrin Cyclodextrin Technologies Inc TRMB-P
Cholesterol Sigma C8667-1G
Mevalonolactone Sigma M4667-1G
IntestiCult™ Organoid Growth Medium (Mouse) Stemcell technologies 6005
Critical Commercial Assays
Prostaglandin E2 Parameter Assay Kit Fisher KGE004B
Amplex® Red Cholesterol Assay Kit Life Technologies A12216
Deposited Data
Microarray data This paper GSE98516
Experimental Models: Organisms/Strains
Mouse: Lpcat3 Floxed mice Rong et al., 2015 N/A
Mouse: VillinCre-ERT2: Tg(Vil-cre/ERT2)23Syr Generated by Sylvie Robine (Institut Curie) and provided by Nicholas Davidson (Washington University) N/A
Mouse: C57BL/6J-ApcMin/J The Jackson Laboratory Stock No: 002020
Mouse: Srebf2 intestinal transgenic mice Ma et al., 2014 N/A
Oligonucleotides
See table S1
Software and Algorithms
GraphPad Prism GraphPad Software https://www.graphpad.com/scientificsoftware/prism/
ImageJ NIH https://imagej.nih.gov/ij/
Other
ProLong® Diamond Antifade Mountant with DAPI Thermo Fisher P36966