Table 1.
Platelet purinergic receptors assessed by RNA sequencing of healthy human or murine platelets.
| Purinergic receptor | Nucleotide/nucleoside | G-Protein coupling | Effect on platelet cAMP | Effect on platelet calcium | Large human platelets (Clancy et al., 2017) (FPKM) | Small human platelets (Clancy et al., 2017) (FPKM) | All human platelets (Rowley et al., 2011) (RPKM) | All murine platelets (Rowley et al., 2011) (RPKM) |
|---|---|---|---|---|---|---|---|---|
| P1 Purinergic receptors | ||||||||
| A1AR | Ado | Gαi | – | – | 0 | 0 | 0.05 | 0 |
| A3AR | Ado | Gαi | – | – | 0 | 1.80 | 0.08 | 1.86 |
| A2aAR | Ado | Gαs | ↑ | – | 0 | 2.23 | 31.80 | 0.36 |
| A2bAR | Ado | Gαs | ↑ | – | 0 | 0 | 5.37 | 0 |
| P2 Purinergic receptors | ||||||||
| P2X1 | ATP | Ion-gated channel | – | ↑ | 4.21 | 1.55 | 90.79 | 630.06 |
| P2Y1 | ADP | Gαq | – | ↑ | 0 | 0 | 12.53 | 30.56 |
| P2Y12 | ADP | Gαi | ↓ | – | 13.19 | 1.77 | 73.75 | 1044.24 |
The two studies summarized here included healthy human donors and isolated platelets by sorting [large and small platelets (Clancy et al., 2017)] or by centrifugation followed by leukocyte depletion (Rowley et al., 2011). The human cohorts, methods of platelet isolation, and RNA extractions are described in Rowley et al. (2011) and Clancy et al. (2017). Mice sequencing data represent RNA transcripts in platelets from male and female C57BL/6 murine models (Rowley et al., 2011). Of note: The data summarized here are derived from platelets from two human male donors (large and small platelets) and from one male and one female donor (data in “all platelet” column). Data validation is required in various individuals of different ages and sexes in order to establish a broader distribution profile of purinergic receptors. cAMP, cyclic AMP; FPKM, fragments per kilobase of transcript per million mapped reads; RPKM, reads per kilobase of transcript per million mapped reads; Ado, Adenosine; ATP, adenosine triphosphate; ADP, adenosine diphosphate.