Fig. 4.
Using FRET to minimize apoptosis and proliferation. The large degree of FRET between SYTO-24 and propidium iodide allows for efficient determination of live versus dead counts. As seen in (A), these DNA stains provide a binary indicator with only positive (green) or negative (red) nuclei. Dying cells are highlighted with arrows. Determining the degree of apoptosis is done by thresholding to the respective wavelengths and quantifying live (B) and dying (C) nuclei, in these frames live=12,249 and dead=158 respectively. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)