Figure 2.
ILC3-Expressed GPR183 Is Required for the Formation of Colonic Lymphoid Tissues
(A) Distribution of GFP+ cells in the small intestine and colon of Gpr183GFP/+ mice. Tissue sections were co-stained with α-CD90.2 and α-B220 Abs. Scale bars (white) represent 100 μm.
(B) Number of CPs and ILFs in the small intestine and colon of Rorc(γt)GFPGpr183+/+ and Rorc(γt)GFPGpr183−/− mice (n = 3–5). The upper panel shows representative images of a CP and an ILF from Rorc(γt)GFPGpr183+/+ mice. Scale bars (red) represent 100 μm.
(C) Number of peripheral and mesenteric lymph node cells (n = 6–8), Peyer’s patches (n = 10), and colonic patches (n = 3) from Gpr183+/+ and Gpr183−/− mice.
(D) Number of RORγt+ clusters in the colon of bone marrow chimeras (n = 5–9). Bone marrow cells from Gpr183+/+ or Gpr183−/− mice were injected into either Gpr183+/+ or Gpr183−/− irradiated recipient mice.
(E) Number of CPs in the colon of Rag1-deficient Gpr183+/+ and Gpr183−/− mice (n = 5).
(F) Number of CPs, ILFs, Peyer’s patches, and colonic patches in Rorc-cre Gpr183flox/flox mice and Gpr183flox/flox or Gpr183flox/+ controls (n = 3–6).
Data are represented as means ± SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001 by Student’s t test or one-way ANOVA with Tukey’s post-test (D). Data are representative of or combined from two (F) or three (A–E) experiments. See also Figures S3 and S4.