Figure 5. Metabolic effect of 2DG treatment in low- and high-grade ccRCC and normal cortex primary cultures.
(A) Glucose quantification performed in conditioned culture medium of normal cortex (n = 3), low- grade (n = 4) and high-grade (n = 3) ccRCC primary cultures treated for 24 hours with 5 mM 2DG. (B) Lactate quantification performed in conditioned culture medium of normal cortex (n = 4), low-grade (n = 3) and high-grade (n = 3) ccRCC primary cultures treated for 24 hours with 5 mM 2DG. (C) Quantification of reduced MTT dye performed by MTT assay in normal cortex (n = 4), low-grade (n = 9) and high-grade (n = 5) ccRCC cultures treated for 72 hours with 5 mM 2DG. (D) Quantification of ATP content in normal cortex (n = 3), low-grade (n = 5) and high- grade (n = 4) ccRCC cultures treated for 24 hours with 5mM 2DG. All data are represented as percentage with respect to corresponding control (untreated) cells considered equal to 100%, except for panel A in which the treated cells are considered equal to 100%. Data expressed as mean ± SEM; *p < 0.05.