Fig. 4. Antibiotic responsive conditional terminators.
The antibiotic-dependent response of known and novel regulators as measured in-vivo by term-seq and RNA-seq. Black, green and blue RNA-seq coverage and term-seq sites denote the control (LB), lincomycin, and erythromycin conditions, respectively. Term-seq sites represent average read coverage across 3 biological replicates. (A) The B. subtilis bmrCD operon. (B) The B. subtilis vmlR gene. (C-F) Antibiotic dependent transcriptional read-through in novel regulators discovered in L. monocytogenes and E. faecalis. (G) Condition-specific read-through calculated in the control and the seven antibiotics exposure experiments. The antibiotic class is defined by the cellular process/component targeted. RNA-seq was normalized as in Fig. 3. Antibiotics and abbreviations used: Lincomycin (Lm), Erythromycin (Em), Chloramphenicol (Cap), Kanamycin (Km), Ofloxacin (Oflox), Bacitracin (Bac) and Ampicillin (Amp).