Figure 3. MiR-384 is a target of CRNDE.
(A) The seed sequence of miR-384 matched the sequence of CRNDE gene. The sequences include the wild type (WT) and mutated (MUT) target sites, the mature miR-384 and the target sequence bound by miR-384. (B) Luciferase report assay of HEK293 cells co-transfected with CRNDE reporter plasmid containing WT sequences and miR-384 mimics or miR-384 inhibitors, or their corresponding negative controls i.e. mimics NC and inhibitors NC, and luciferase reporter activity was suppressed by miR-384 mimics transfection and increased by miR-384 inhibitors transfection. (C) Luciferase report assay of HEK293 cells co-transfected with CRNDE reporter plasmid containing MUT sequences and miR-384 mimics or miR-384 inhibitors, or their corresponding negative controls, and luciferase reporter activity was not affected by miR-384 mimics transfection and miR-384 inhibitors transfection. (D) The BCPAP cells transfected with CRNDE overexpressing vector showed a decreased expression of miR-384 in BCPAP cells compared with control group (NC). (E) The K1 cells transfected with CRNDE siRNA showed an increased expression of miR-384 compared with control group (siRNA NC). N = 4, *P<0.05, **P<0.01.