Table 2.
NAD(P)(H) Concentrations (μM) in HCT116 Cells and Selected Mouse Tissues, Based on Extraction Using the 40:40:20 with 0.1 M Formic Acid Method
| HCT116 | Brain | Heart | Lung | Liver | Skeletal muscle | |
|---|---|---|---|---|---|---|
| NADPH | 49.7 (±3.8) | 25.4 (±2.5) | 21.8 (±5.4) | 31.7 (±16.7) | 244.0 (±12.4) | 9.3 (±1.8) |
| NADP+ | 4.9 (±0.2) | 6.8 (±1.4) | 45.0 (±0.4) | 69.6 (±7.0) | 45.1 (±10.6) | 4.7 (±0.3) |
| NADH | 75.4 (±2.8) | 8.5 (±0.4) | 77.7 (±23.4) | 0.5 (±0.2) | 162.9 (±9.3) | 8.8 (±5.7) |
| NAD+ | 439.4 (±15.8) | 178.5 (±17.9) | 369.6 (±20.2) | 95.2 (±15.7) | 429.2 (±8.2) | 277.9 (±3.4) |
The results are the average of three biological replicates for cell samples, and three pieces of tissue from the same organ of one mouse for tissue samples. The error is the SD.