Figure 4.
The number and area fraction of Rab5a- and CatD-immunoreactive compartments are increased in the EC of aged APOE4/4 vs. APOE3/3 mice. Rab5a-immunoreactive early endosomes and CatD-immunoreactive lysosomes were quantified in the entorhinal cortex of 18 month-old APOE3/3 and APOE4/4 mice. (A) Representative images showing Rab5a and CatD immunolabeling (red), co-stained with Nissl (green). Black and white images were generated in ImageJ and used for quantification. (B–D) Quantification of average Rab5a- and CatD-positive compartment number (B), size (C), and area fraction (D) in APOE4/4 vs. APOE3/3 mice. (*p < 0.05; ***p < 0.001).