Fig. 2.

Comparison of TLR expression by pDC and mDC from healthy young and healthy elderly subjects. Fourteen healthy young (mean age, 34.3 years) and 17 healthy elderly subjects (mean age, 72.6 years) were recruited for testing the TLR-2 and TLR-4 expression on cells surface of mDC. The intracellular expression of TLR-7 and TLR-9 in pDC were compared between 13 young (mean age, 26.1 years) and 13 elderly (mean age, 69.4 years) subjects. PMBC isolated from these subjects were stained with lineage markers (FITC), HLA-DR (PerCP), CD11c (APC), and TLR (PE) of interest. Data shown are mean values from each age group with standard errors shown in error bars. *p ≤ 0.05.