Figure 1. Induction of LAIR-1 in T cells.
(Panel A) Naïve DBA/1 splenocytes were cultured with α1(II) for 24 hours. The expression of LAIR-1 was examined by flow cytometry using a PE conjugated anti-LAIR-1 antibody. Histograms are gated on CD4+ T cells. The data are representative of three separate experiments. Mean fluorescence (MF) for cells cultured with media alone (light grey line) was 392±32 while MF for cells cultured with α1(II) (black line) was 1048 ± 45, p ≤ 0.001
(Panel B) Murine T hybridoma cells (2×106/ml) were stimulated with type II collagen (α1(II), 50 µg/ml) for the indicated time period. The expression of LAIR1 was examined by flow cytometry as in Panel A. Mean fluorescence = 1208 ± 53 compared to 185 ± 24 as a mean of three separate experiments.
(Panel C). Induction of LAIR1 by type II collagen. Murine T hybridoma cells (1×107/10ml) were stimulated with α1(II) (50 µg/ml) for the indicated time period. Samples were loaded and expression level of LAIR1 was examined with Western blot analysis using anti-LAIR-1. Actin was used as control. Data shown are representative of three separate experiments.