Figure 3.
eIF2α-NTD surfaces affected by binding to eIF2Bα. (A) TROSY-HSQC spectra of 2H/15N-labeled eIF2α-NTD, free (black) and in the presence of excess unlabeled eIF2Bα (red). (B) The boxed area in (A). Some of the peaks experiencing selective signal loss are labeled. (C) Plot of signal loss for residues in eIF2α-NTD. Gray bars represent indeterminate residues for which no analysis could be performed. (D) Residues experiencing selective signal loss mapped onto the structure of eIF2α-NTD, colored from yellow (>1σ) to red (>3σ). Indeterminate residues are colored gray. Residues <1σ are colored black.