Figure 2. Diversity of the fecal microbiota in mice.
Microbiota from the following groups of mice were analyzed: Naïve-CD44WT (NaWT; n=1), EAE-CD44WT (EAEWT; n=3), Naïve-CD44KO (NaKO; n=1) and EAE-CD44KO (EAEKO; n=3). For EAE induction, mice were immunized with MOG35-55 peptide to induce EAE along with 200 ng of pertussis toxin (day 0) and 400 ng of pertussis toxin (day 2). (A) shows the violin plots with median and quartile ranges of the Shannon Index in each group (B) shows the box-and-whisker plot for the probability of two individuals taken at random from each group belonging to the same OTU (C) shows the Circos plot representing the phylum-level taxonomic classification of OTUs in each group. From the outside to the inside track: OTUs are arranged by percentage abundances. Colors in tracks represent each phyla belonging to each group. (D) Principal components analysis (PCA) shows a close relationship between the Naïve and EAEKOs, both of which are distinct from the naïve and EAEWTs. The percentage of variation between the groups was explained by the principal coordinates indicated on the axis.