Table 2.
Pore diameters and reaction rates extrapolated to N = 0
| Mutant | Pore diameter, D, derived from scaling analysis (Å) | Second-order rate constant, k (M−1⋅s−1), pH 8.5 |
|---|---|---|
| T129C7 | 23 ± 1 | 25 ± 2 |
| L135C7 | 21 ± 1 | 160 ± 20 |
| T117C7 | 20 ± 1 | 970 ± 30 |
| M113C7 | 20 ± 0 | 22 ± 3 |
| E111C7 | 16 ± 0 | 930 ± 10 |
| S106C7 | 27 ± 2 | 10 ± 2 |
| K8C7 | 21 ± 2 | 430 ± 30 |
The values were obtained from plots of ln k′ versus N as described in the text. The data for the plots were derived from experiments in which MePEG-OPSS was added to the trans side of the bilayer, with the exception of S106C7 and K8C7, where MePEG-OPSS was added to the cis side of the bilayer. The conditions are in Fig. 2 (legend). The values are averages ± SD from at least four experiments.