Figure 5. Quercetagetin inhibits Pim-1 activity but not its expression in HG-treated VSMCs.
A. qRT-PCR was used to detect Pim-1 mRNA expression in HG-treated VSMCs. B. Western blotting was used to detect Pim-1, Bad and p-Bad(T112) protein expression in HG-treated VSMCs. For qRT-PCR and western blotting, VSMCs were maintained in media supplemented with HG (25 mmol/L D-glucose), HG+Quer (25 mmol/L D-Glucose + 5.5 μmol/L quercetagetin), or HG+DMSO (25 mmol/L D-Glucose + 0.3% (v/v) DMSO) for 48 hours, then cells were harvested and subjected to analysis. C. Levels of Pim-1 protein in HG-treated VSMCs. D. Ratio of p-Bad/Bad in HG-treated VSMCs. **P < 0.01 vs. HG or HG+DMSO.