FIGURE 1.
Effect of Ligustilide (LIG) on memory impairment and neuronal loss in amyloid precursor protein (APP)/PS1 transgenic mice. C57 mice were treated with vehicle control and APP/PS1 mice treated with either vehicle control or LIG (5 μM) for 14 weeks starting at 8.5 months of age. (A) Effect of LIG on Y-maze test. LIG treatment significantly increased the percentage of alternations used as an index of short-term memory. (B) Effect of LIG on the passive avoidance test. LIG treatment increased the step-down latency in comparison to vehicle-treated APP/PS1 mice. (C) Representative immunostaining of NeuN in the hippocampal CA1 region and cerebral cortex. Scale bar, 25 μm. (D) Quantitative image analysis of NeuN based on the integrated optical density (IOD) of positive immunostaining. The data are expressed as mean ± SEM; n = 12 for behavior tests, n = 4 for immunohistochemistry; ∗p < 0.05, ∗∗p < 0.01.