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. Author manuscript; available in PMC: 2017 Oct 26.
Published in final edited form as: Nano Lett. 2017 Oct 2;17(10):6131–6139. doi: 10.1021/acs.nanolett.7b02716

Figure 1. DNA-Exchange-Imaging.

Figure 1

Distinct targets (T1, T2, …, Tn) are labeled using corresponding antibodies conjugated to orthogonal DNA docking strands (P1, P2, …, Pn) in a single step. Imager strands (P1*, P2*, …, Pn*) are sequentially introduced to visualize target signals. The imager strands are washed away rapidly using low ionic strength buffer after each round of imaging. After imaging, all images are computationally registered and a final merged image is reconstructed by assigning pseudo-colors to each target image.