Extended Data Figure 3. Dyn2 depletion does not phenocopy starvation-induced inhibition of Drp1.
a,b, The phosphorylation status of Drp1 was evaluated by immunoblot on whole-cell lysates in scrambled and Dyn2 siRNA-treated cells using antibodies against (a) phosphoserine637-Drp1 and (b) phosphoserine616-Drp1. Antibodies against Drp1 and Dyn2 were used to measure total Drp1 and Dyn2 levels, respectively, and anti-GAPDH was used as a loading control. The optical densities of phosphorylated-serine Drp1 signal were normalized to their corresponding GAPDH signal (graphs in a,b). The data represented in graphs for both (a) and (b) were obtained from three biological replicate experiments. Error bars represent the s.e.m. *p<0.01 statistical significance via analysis by paired t-Test.