Figure 6. WNT Inhibition Restores Angiogenic Deficits in HD iBMECs and Model of NVU Impairment in HD.
(A) Wound-healing assay shows HD iBMECs treated with 20 μM XAV939 have decreased migration into the wound. The plot shows change in area overtime. [(n) = 3 (all lines) independent experiments/differentiations and adjusted p value; (33Qv66Q 4.43 × 10−2), (33Qv71Q 2.27 × 10−6), (33Qv109Q 5.31 × 10−5), (66Qv66Q-XAV 5.70 × 10−3), (71Qv71Q-XAV 1.34 × 10−4), and (109Qv109Q-XAV 2.96 × 10−3); two-way ANOVA and bonferroni post hoc].
(B) Model of EC barrier dysfunction in HD. Schematic diagram of NVU in HD and selected genes (yellow denotes genes upregulated and blue downregulated in HD) that are changed in HD iBMECs and HD astrocytes. These genes may contribute to neuronal dysfunction and death. # (*p < 0.05; **p < 0.01; and ***p < 0.001).
For all of the error bars (mean ± SEM).