FIGURE 6.
Intracellular localization of A. baumannii in epithelial cells. Semi-confluent A549 cell monolayers were infected as reported before. At 12-h post-infection, cells were fixed and actin was stained with rhodamine-conjugated phalloidin (red), while nuclei and bacterial DNA were stained with DAPI (blue). Images, representatives of at least three independent experiments, in duplicate, were captured using a Leica camera and processed using Qwin software (Leica). Arrows point representative intracellular bacteria. Uninfected cells were used as control (CC). Scale bar, 20 μm.