FIGURE 5.
FISH for Arc, Egr3, Egr4, H1a, pri-miR132, Sox11, and Ptgs2. Rats were stimulated with pp-HFS and tissue was taken at the 4 h time point followed by FISH with transcript specific riboprobes. A 2 h time point is included for pri-miR132. Each slice was imaged at two different magnifications; zoom-out (top) and zoom-in (bottom). Control DG (right; c = control) and the stimulated DG (left; s = stimulated). The mean optical density of the in situ signal from the ML, GCL and background was quantified. (∗p < 0.05, ∗∗p < 0.005, #p < 0.001) ns, not significant. Error bars represent SEM. n = 3 per group. Zoom-out Scale Bar: 200 um. Zoom-in Scale Bar: 100 um.