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. 2017 Sep 6;6:e26423. doi: 10.7554/eLife.26423

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© 2017, Marçais et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 3. — (A) Percentage (mean +SD) of CD107a⁺ cells among splenic CD11b^lo NK cells of the indicated subset from Ncr1^iCre/+ or Ncr1^iCre/+ Mtor^lox/lox mice following 4 hr stimulation with coated anti-NK1.1 or YAC-1 cells (n = 5 mice of each genotype in three independent experiments, t-tests comparing each subset in both genotype, *p<0.05, **p<0.01, n.s. non significant). (B) Percentage (mean +SD) of IFN-γ⁺ or CD107a⁺ cells among splenic NK cells of the indicated subset from C57BL/6 or B2m^−/− mice following 4 hr stimulation with coated anti-NK1.1 in the presence or absence of 250 nM Torin2 (n = 9–10 mice of each genotype in five independent experiments, two-way ANOVA comparing each subset to its counterpart in B2m^−/− mice, *p<0.05, **p<0.01, ***p<0.001, n.s. non significant). (C) Percentage (mean +SD) of dead YAC-1 cells after a 4 hr co-culture with purified NK cells of the indicated genotype at the indicated Effector/Target ratio in the presence or absence of 250 nM Torin2 (n = 9 C57BL/6 and 7 B2m^−/− mice in four independent experiments, two-way ANOVA comparing each E/T ratio of C57BL/6 + Torin2 to C57BL/6, B2m^−/− or B2m^−/− +Torin2 as indicated by the color, ***p<0.001, n.s. non significant). (D) Percentage of remaining B2m^−/− target cells following in vivo cytotoxicity experiment as described in the Materials and methods. Each dot represents a single mouse, bars indicate mean and SD (n = 9 control treated mice and 7 Torin2 treated mice in two independent experiments, t-test, ***p<0.001).

Figure 3—figure supplement 1. — (A) Percentage (mean +SD) of CD107a⁺ cells among splenic CD11b^lo NK cells of the indicated subset from Ncr1^iCre/+ or Ncr1^iCre/+ Mtor^lox/lox mice following 4 hr stimulation with coated anti-NK1.1 or YAC-1 cells (n = 5 mice of each genotype in three independent experiments, t-tests comparing each subset in both genotype, *p<0.05, **p<0.01, n.s. non significant). (B) Percentage (mean +SD) of IFN-γ⁺ or CD107a⁺ cells among splenic NK cells of the indicated subset from C57BL/6 or B2m^−/− mice following 4 hr stimulation with coated anti-NK1.1 in the presence or absence of 250 nM Torin2 (n = 9–10 mice of each genotype in five independent experiments, two-way ANOVA comparing each subset to its counterpart in B2m^−/− mice, *p<0.05, **p<0.01, ***p<0.001, n.s. non significant). (C) Percentage (mean +SD) of dead YAC-1 cells after a 4 hr co-culture with purified NK cells of the indicated genotype at the indicated Effector/Target ratio in the presence or absence of 250 nM Torin2 (n = 9 C57BL/6 and 7 B2m^−/− mice in four independent experiments, two-way ANOVA comparing each E/T ratio of C57BL/6 + Torin2 to C57BL/6, B2m^−/− or B2m^−/− +Torin2 as indicated by the color, ***p<0.001, n.s. non significant). (D) Percentage of remaining B2m^−/− target cells following in vivo cytotoxicity experiment as described in the Materials and methods. Each dot represents a single mouse, bars indicate mean and SD (n = 9 control treated mice and 7 Torin2 treated mice in two independent experiments, t-test, ***p<0.001).