Fig. 3.
RAN action on proliferative myoblasts: 48–72 h. Intracellular calcium signaling and p70S6 kinase play an important role in the early stages of myogenesis. We studied whether RAN could act on CaMKII protein expression and p70S6 activation during proliferation phase. a Immunofluorescence analysis and quantification (20X): RAN increased the signal of CaMKII protein expression compared with GM at 48 h. b Phalloidin staining and phase contrast images corroborated the morphological changes observed in proliferative myoblasts (Fig. 2). c Western blot analysis: RAN significantly increased MyoD and p21 protein content at 72 h of proliferation. d Western blot analysis: RAN did not induce myoblast proliferation activating p70S6 kinase at 72 h. Representative immunoblots are shown. Objective: 20X. Anova test: >p ≤ 0.05, t test: *p ≤ 0.05 or **0.01 vs. GM