Figure 2.
Increased expression of SOX2, AXIN2, and DKK1 in MCF-7 breast cancer cells with short-term 4-hydroxytamoxifen treatment. The expression of (A) SOX2, (B) AXIN2, and (C) DKK1 relative to MCF-7 control measured by quantitative RT-PCR in MCF-7 breast cancer cells exposed to 4-hydroxytamoxifen (100 nM) for 2–5 days, relative to the MCF-7 parental line. (D) Western blot analysis showing expression of SOX2 in 4-hydroxytamoxifen (TAM; 100 nM)-treated MCF-7 cells. Tubulin is the loading control.