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. Author manuscript; available in PMC: 2018 Mar 6.
Published in final edited form as: Nature. 2017 Sep 6;549(7670):86–90. doi: 10.1038/nature23454

Figure 2. Asymmetric L/R Prrx1a expression drives heart looping in zebrafish.

Figure 2

(a) Cell lineage tracing, n=4. The heart tube is revealed by MF20 immunostaining. (b) Snapshots extracted from videos of control and prrx1a morphant Tg(tbx5a:eGFP) embryos. Red and magenta represent right and left cardiomyocyte precursors. (c) Prrx1 expression in a LifeAct injected Tg(actb2:myl12.1-mCherry) embryo to visualize F-actin and myosin II, respectively. Star, mesenchymal cell; arrows, epithelial cells. (d) Maximum intensity projection of a Tg(tbx5a:eGFP) embryo. Bracket highlights the “cable-like” structure. (e) Photoablation (Pa) in Tg(tbx5a:eGFP) embryos. Ventricles (V) and atria (A) are shown in transverse sections on the right. (f) High resolution images of the “cable-like” structure analyzed as in (c). (g) Palladin (Palld), Prrx1 and tbx5a expression in control and prrx1a morphant embryos. Scale bars 50 µm except for (c) and high power images in (a, f and g), 10 µm. P, pericardium.