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Published in final edited form as: Nat Immunol. 2016 Dec 19;18(2):173–183. doi: 10.1038/ni.3646

Induction of autoimmunity by T cell–specific deletion of Satb1. (a) Hematoxylin and eosin staining of tissue sections from 16-week-old Satb1^fl/+Cd4-Cre⁺ mice (n = 4), Satb1^fl/flCd4-Cre⁺ mice (n = 10) and Satb1^fl/flCd4-Cre⁺ mice after 1 × 10⁶ wild-type (WT) T_reg cell transfer on day 4 after birth (n = 10). Scale bars, 200 μm. (b) Severity of disease in mice shown in a. Horizontal lines indicate mean. ns, P > 0.05; *P ≤ 0.05; **P ≤ 0.01 (Kruskal–Wallis test followed by Dunn’s multiple comparisons test). (c) Oophoritis and insulitis score of donor Satb1^fl/flCd4-Cre⁺ mice and Rag2^−/− mice transferred with splenocytes from donor. Each recipient received cells from each donor. Horizontal lines indicate means. (d) Percentages of IFN-γ-producing cells among peripheral CD4⁺ non-T_reg cells in mice described in a. Horizontal lines indicate means (n = 3, 5 and 6 for Satb1^fl/+Cd4-Cre⁺, Satb1^fl/flCd4-Cre⁺ and T_reg-transferred Satb1^fl/flCd4-Cre⁺ mice, respectively). ****P ≤ 0.0001 (ordinary one-way ANOVA, followed by Holm–Sidak multiple comparisons test). (e) Relative levels (arbitrary units (a.u.)) of serum IgE in mice shown in a (n = 8 per group). Horizontal lines indicate means. **P ≤ 0.01 and ***P ≤ 0.001 (ordinary one-way ANOVA, followed by Holm–Šídák multiple comparisons test). Data are representative and summary of 4, 10 and 10 experiments for Satb1^fl/+Cd4-Cre⁺, Satb1^fl/flCd4-Cre⁺ and Satb1^fl/flCd4-Cre⁺ with WT T_reg cell transfer independent experiments (a,b), summary of 4 or 5 independent experiments (c) or summary of 3 independent experiments (d,e).