Figure 4.
Potential roles of Satb1 in activating Treg-SEs. (a) Percentage of Treg-SEs bound by Satb1 in DP, imCD4SP, a mixture of tTreg precursor (pre-tTreg) and thymic Treg (thyTreg) cells, and peripheral Treg cells. (b) Chromatin accessibility and H3K27ac changes during Treg cell development around sites where Satb1 bound at the DP stage in open or closed chromatin within Treg-SEs. Average signal intensity of Satb1-binding sites (172 and 65 regions at open and closed chromatin, respectively) is shown for indicated cell types. (c) Density of indicated transcription factor binding in DP and peripheral Treg cells around Satb1-binding sites at the DP stage in open or closed chromatin within Treg-SEs. Normalized ChIP-seq signal density is plotted for Satb1-binding sites ± 2 kb. (d) Binding of Satb1 during tTreg cell development and chromatin accessibility and transcription factor binding in DP and Treg cells at the Treg-SE of the Foxp3 locus. Sequence conservation among vertebrates is also shown. The transcription start site (TSS) of the Foxp3 gene and CNS are highlighted. Scale bar, 5 kb. Histone ChIP-seq data are representative of 2 independent experiments (b,d) and transcription factor ChIP-seq and ATAC-seq data are from 1 experiment (a–d).