Figure 3.
Satb1 expression in Treg precursor cells and binding to Treg-SEs. (a) Differential expression of genes encoding epigenetic modifiers in tTreg precursor (pre-tTreg) and peripheral Treg cells. Epigenetic modification–associated genes that are upregulated and downregulated in pre-tTreg cells are highlighted in red and blue, respectively, and those that show similar expression are shown in black. (b) Relative expression of epigenetic modifiers upregulated in pre-tTreg cells throughout thymocyte development and in various immune cells. Those with uniform gene expression patterns (variance < 0.5) were excluded, and row z-score is shown in heat map. (c) Relative mRNA expression of Satb1 and Foxp3 during Treg cell development. ETP, early T cell lineage progenitor; ISP, immature SP; DPbl, DP blasts; DPsm, small DP; HSC, hematopoietic stem cell; MPP, multipotent progenitor; CLP, common lymphoid progenitor. Average normalized values from 2 independent RNA-seq experiments (a,c) and averages of 3 independent microarray experiments for each cell type (b) are shown.