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. Author manuscript; available in PMC: 2018 Jan 13.
Published in final edited form as: Nature. 2017 Jul 5;547(7662):217–221. doi: 10.1038/nature22991

Figure 4. Vaccine-induced T cells demonstrate broad shifts in their transcriptional programs; the repertoire of neoantigen-specific T cells persists and broadens following PD-1 blockade.

Figure 4

a, b, Ex vivo class II tetramer staining of Patient 4 CD4+ T cells. Visualization of transcriptomes of single CD4+ T cells pre-vaccination and tetramer-positive CD4+ cells post-vaccination by tSNE. c, Summary of neoantigen-specific T cell responses after vaccination and after PD-1 blockade for Patients 2 and 6. Example IFN-γ ELISPOT results are shown for week 16 post-vaccination and post-PD-1 CD4+ T cells against mut-CDK13, -JPH1, and –ADM2d for Patient 2. For complete response kinetics, see Extended Data Fig. 8a.