Figure 7.
Tight junctions are functionally impaired in K14-Cre/EcadFl/− mice. (A) Inside-out permeability assay. Skin sections of control and mutant newborn mice intradermally injected with biotin were stained with streptavidin to follow the penetration of biotin (green) and counterstained with occludin to mark the tight junctions present in the granular layer. (B) Impedance measurements of ventral skin biopsies isolated from newborn control and E-cadherin mutant mice. Typical experiments are depicted in Nyquist plots; numerical values represent mean±s.e.m. *P-value=0.03. N=8 for control and N=4 for K14-Cre/EcadFl/− (−/−) mice.