Fig. 7.
Expression of a CDCP1-blocking fragment, ECC, increases LD abundance and reduces metastasis. (A) Representative images of mice with luciferase-labeled UCI-082014 (i) or MDA-MB-231 (ii) tumors at week 5 and 8 posttumor implantation, respectively. *VC-5 killed 2 wk early because of extensive disease progression and visibly reduced quality of life. (B) ECC reduces primary (1°) tumor growth of UCI-082014 (i) and trends toward reducing 1° tumor growth of MDA-MB-231 (ii) cells implanted into the fat pad of Rag2−/− mice. (C) ECC expression reduces lung metastasis of UCI-082014 and MDA-MB-231 tumors. (i and ii) Representative 4× images of H&E-stained lungs from mice implanted with UCI-082014 (i), metastases are shown by arrowheads, and MDA-MB-231 (ii) cells. (iii and iv) Quantitation of UCI-082014 (iii) and MDA-MB-231 (iv) metastasis. (D) ECC reduces CDCP1 dimer formation in 1° tumors as assessed by PLA. Representative 20× images (i) and quantitation (ii) of PLA. (E) ECC increases LD abundance in 1° tumors. Representative 20× images (i) and quantitation (ii) of Oil Red O (ORO) staining. (F) VC-transduced 1° tumors have higher LD abundance than the corresponding metastatic nodules. Representative 20× images (i) and quantitation (ii) of ORO staining. (G) ECC stimulates ACSL activity in 1° tumors. Quantitation of ACSL activity in VC- and ECC-transduced UCI-082014 and MDA-MB-231 primary tumor tissue. Each point represents an individual tumor. P values (as indicated) analyzed by one-way ANOVA with multiple comparison post hoc t test and error bars represent SEMs. A.U., arbitrary units.