Fig. 1.
Humoral immune response in one chimpanzee (Ch1422, strain HC-TN) with acute resolving HCV infection that developed NtAb. Qualitative detection of HCV RNA in RT-nested PCR is indicated (on top) by red bars. Detection of antibody to core and nonstructural proteins by using the anti-HCV 2.0 assay is indicated (on top) by green bars. The shaded area indicates HCV genome titers as determined by Roche Monitor 2.0; values below the detection limit of ≈500 genome equivalents per ml are shown as 0. The blue, green, and orange lines and the red bars correspond to levels of anti-E1, anti-E2, anti-HVR1, and NtAb, respectively. Percentage of neutralization was calculated by comparison with a preinoculation sample from the same animal. Neutralization values ≤1 are indicated as equal to 1.