Fig. 3.
Loss of DJ-1 confers susceptibility to MPTP-induced neuronal death. (A–D) DJ-1+/– and DJ-1–/– mice were treated with MPTP or saline (control) as for Fig. 2, and sections of the SNc at the level of the medial terminal nucleus were prepared 14 days later. Sections were immunostained to detect TH+ neurons. Shown are the SNc of saline-treated DJ-1+/+ (A) and DJ-1–/–(B) mice and SNc of MPTP-treated DJ-1+/+ (C) and DJ-1–/–(D) mice. (E and F) Quantification of SNc neurons in the mice in A–D using either TH staining (E) or cresyl violet staining (F). Data in E and F are presented as mean ± SEM (n = 5–7 animals per group), where * and ** denote significance levels (ANOVA) of P < 0.05 and P < 0.01, respectively.