Figure 3.
ApoE KO SCA-1+ Cells Show a Higher Migration toward the Inner Vascular Wall in Comparison with WT Counterparts
(A) Montage microphotographs. WT and ApoE KO AdvSCA-1+ cells were transduced with a nuclear RFP-inducing lentiviral construct. 1 × 106 cells in Matrigel plugs were transplanted to the outer side of the vascular wall of femoral arteries, in WT and ApoE KO 6-month-old animals. Cells were allowed to migrate for 24 and 72 hr prior to euthanizing the mice and assessing them with en face immunolabeling, using a VE-cadherin (green) antibody to mark the endothelial cell layer. DAPI is blue.
(B) Quantitative and spatial analysis of RFP+ cells migrating from the adventitia to the lumen from the outer vascular wall after 24 and 72 hr. n = 3 transplantation experiments, geometric means, error bars with 95% CI. ∗p < 0.05, ∗∗p < 0.01.