Table 2.
Sequences of the couples of primers used for the amplification of the genes.
| Primers | Sequences |
|---|---|
| HLA-DR3 | Fw: 5′CACGTTTCTTGGAGTAC3′ Rv: 5′CGTAGTTGTGTCTGCAGTAGT3′ |
| HLA-DR4 | Fw: 5′CAGGTTAAACATGAGTGTCATTTCTTAAAC3′ Rv: 5′GCTGTCGAAGCGCACGTACTCCTCTTGGTG3′ |
| HLA-DQA1∗05:01 | Fw: 5′ACGGTCCCTCTGGCCAGTA3′ Rv: 5′AGTTGGAGCGTTTAATCAGAC3′ |
| HLA-DQB1∗02:01 | Fw: 5′GTGCGTCTTGTGAGCAGAAG3′ Rv: 5′GCAAGGTCGTGCGGAGCT3′ |
| HLA-DQB1∗06:02 | Fw: 5′CGTGCGTCTTGTGACCAGAT3′ Rv: 5′GCTGTTCCAGTACTCGGCAT3′ |
| HLA-DQB1∗03:02/3 | Fw: 5′GACGGAGCGCGTGCGTTA3′ Rv: 5′AGTACTCGGCGTCAGGCG3′ |
| HLA-DQB1∗03:03 | Fw: 5′GACGGAGCGCGTGCGTTA3′ Rv: 5′CTGTTCCAGTACTCGGCGT3′ |
Fw: forward; Rv: reverse.