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. Author manuscript; available in PMC: 2017 Aug 2.
Published in final edited form as: Nature. 2008 May 14;454(7200):104–108. doi: 10.1038/nature06969

Figure 1. LacZ expression in Tbx18:nlacZ knock-in mice recapitulates endogenousTbx18 expression.

Figure 1

a–d, Section RNA in situ hybridization (ISH) of Tbx18 in mouse embryos (E9.0–E11.5). Tbx18 is expressed very early in the proepicardium (a) and epicardium (b–d). For parallel whole-mount RNA ISH, see Supplementary Fig. 2. e–g, X-gal staining on cardiac sections from Tbx18:nlacZ embryos at E9.5–E11.5. Tbx18:nlacZ cells are detected in the early epicardium at E9.5 (e) and in all epicardial cells covering the heart after E10.5 (f, g, data not shown for E12.5–E13.5). X-gal staining on Tbx18:nlacZ mouse tissues is consistent with Tbx18 mRNA ISH. For the Tbx18:nlacZ targeting strategy, see Supplementary Fig. 3. Black arrows indicate Tbx18 mRNA expression (a–d) and Tbx18:nlacZ cells (e–g); red arrows in c and d indicate that Tbx18 is not expressed in heart between E9.5 and E11.5. For e–g, lower panels are high-magnification views of the upper panels in the heart (black arrow regions). LA/RA, left/right atrium; LV/RV, left/right ventricle; OT, outflow tract; and SV, sinus venosus.