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Migration assay of glioma cell lines on collagen IV. A, vimentin-immunostained GL15 and 8-MG spheroids, after 4 days of culture on collagen IV without (upper row) and with (lower row) addition of CBX, (x50). B, (S - S0) / S0 ratios (mean values ± SEM) obtained from 10 to 30 spheroids per group and condition. Addition of CBX enhanced spheroid disintegration and centrifugal dispersion of migrating cells. ***, p < 0.001.
